Figure 1.

Characterization of E13.5 cortical precursor cultures. Cells were incubated for 3 (a, b, d, e) or 24 h (c and f) and examined by phase (a and d) or brightfield microscopy to detect neuronal markers, immunoreactive MAP2 (b and c), and NSE (e and f). Neuronal markers increase from ≈50% at 3 h (b and e) to ≈90% at 24 h (c and f) (positive staining indicated by arrows; arrowhead = negative cells). Cells were unstained when primary antibody was omitted. MAP2 and NSE analyses were performed on five and two experiments, respectively. (Bar = 50 μm.)