Figure 4.

Localization of PDE1C2, ACIII, and PDE4A in olfactory neuronal tissue. (A, C, and E) Immunohistochemical localization of PDE1C2, ACIII, and PDE4A in olfactory neurons, respectively. (B, D, and F) Protein and peptide competition controls of PDE1C2, ACIII, and PDE4A, respectively. The localization of PDE1C2 and ACIII is found exclusively in olfactory cilia. PDE4A is completely absent from cilia but found throughout the rest of the neuron with high expression levels in axons. Competition controls show complete block of antisera binding verifying the specificity of the reactions. Arrow, cilia layer; AB, axon bundles; ONL, olfactory neuronal layer. (Bar = 50 μm.)