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Comparison of mRNA localizations for nNOSa and total nNOS. cRNA digoxygenin in situ hybridization employed probes directed against nNOS C-terminal (C-term), representing total nNOS mRNA, and an nNOSα-specific probe (ex2) in wt and nNOSΔ/Δ (KO) animals. VCN, ventral cochlear nucleus; PPN, pedunculopontine nuclei; Str, striatum; Ctx, cerebral cortex. (×100.)
