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. 1987 May;53(5):1181–1182. doi: 10.1128/aem.53.5.1181-1182.1987

Elevated temperature method for recovery of Vibrio cholerae from oysters (Crassostrea gigas).

A DePaola, C A Kaysner, R M McPhearson
PMCID: PMC203830  PMID: 3606095

Abstract

Of 222 Vibrio cholerae isolates from diverse clinical and environmental sources, 219 produced visible growth in alkaline peptone broth when incubated overnight at 42 degrees C. In field trials conducted to compare enrichment at incubation temperatures of 42 and 35 degrees C, significantly higher rates of isolation (P less than 0.05) and recovery (P less than 0.01) of V. cholerae from oysters were observed at 42 degrees C.

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Selected References

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  1. Blake P. A., Allegra D. T., Snyder J. D., Barrett T. J., McFarland L., Caraway C. T., Feeley J. C., Craig J. P., Lee J. V., Puhr N. D. Cholera--a possible endemic focus in the United States. N Engl J Med. 1980 Feb 7;302(6):305–309. doi: 10.1056/NEJM198002073020601. [DOI] [PubMed] [Google Scholar]
  2. Colwell R. R., Seidler R. J., Kaper J., Joseph S. W., Garges S., Lockman H., Maneval D., Bradford H., Roberts N., Remmers E. Occurrence of Vibrio cholerae serotype O1 in Maryland and Louisiana estuaries. Appl Environ Microbiol. 1981 Feb;41(2):555–558. doi: 10.1128/aem.41.2.555-558.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gangarosa E. J., Dewitt W. E., Huq I., Zarifi A. Laboratory methods in cholera: isolation of Vibrio cholerae (el tor and classical) on TCBS medium in minimally equipped laboratories. Trans R Soc Trop Med Hyg. 1968;62(5):693–699. doi: 10.1016/0035-9203(68)90121-1. [DOI] [PubMed] [Google Scholar]
  4. Hood M. A., Ness G. E., Rodrick G. E. Isolation of Vibrio cholerae serotype O1 from the eastern oyster, Crassostrea virginica. Appl Environ Microbiol. 1981 Feb;41(2):559–560. doi: 10.1128/aem.41.2.559-560.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KOBAYASHI T., ENOMOTO S., SAKAZAKI R., KUWAHARA S. [A NEW SELECTIVE ISOLATION MEDIUM FOR THE VIBRIO GROUP; ON A MODIFIED NAKANISHI'S MEDIUM (TCBS AGAR MEDIUM)]. Nihon Saikingaku Zasshi. 1963 Nov;18:387–392. doi: 10.3412/jsb.18.387. [DOI] [PubMed] [Google Scholar]
  6. Kaper J., Lockman H., Colwell R. R., Joseph S. W. Ecology, serology, and enterotoxin production of Vibrio cholerae in Chesapeake Bay. Appl Environ Microbiol. 1979 Jan;37(1):91–103. doi: 10.1128/aem.37.1.91-103.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kenyon J. E., Piexoto D. R., Austin B., Gillies D. C. Seasonal variations of Vibrio cholerae (non-O1) isolated from California coastal waters. Appl Environ Microbiol. 1984 Jun;47(6):1243–1245. doi: 10.1128/aem.47.6.1243-1245.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Morris J. G., Jr, Wilson R., Davis B. R., Wachsmuth I. K., Riddle C. F., Wathen H. G., Pollard R. A., Blake P. A. Non-O group 1 Vibrio cholerae gastroenteritis in the United States: clinical, epidemiologic, and laboratory characteristics of sporadic cases. Ann Intern Med. 1981 May;94(5):656–658. doi: 10.7326/0003-4819-94-5-656. [DOI] [PubMed] [Google Scholar]
  9. Twedt R. M., Madden J. M., Hunt J. M., Francis D. W., Peeler J. T., Duran A. P., Hebert W. O., McCay S. G., Roderick C. N., Spite G. T. Characterization of Vibrio cholerae isolated from oysters. Appl Environ Microbiol. 1981 Jun;41(6):1475–1478. doi: 10.1128/aem.41.6.1475-1478.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Weissman J. B., DeWitt W. E., Thompson J., Muchnick C. N., Portnoy B. L., Feeley J. C., Gangarosa E. J. A case of cholera in Texas, 1973. Am J Epidemiol. 1974 Dec;100(6):487–498. doi: 10.1093/oxfordjournals.aje.a112061. [DOI] [PubMed] [Google Scholar]

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