Abstract
Two rumen-cannulated wether sheep were fed a diet containing 1 kg of a liquid-molasses mixture, 80 g of soybean oil meal, and 100 g of chopped wheat straw once a day. In 6 weeks and thereafter, the microbiota adapted such that Quin's oval, a very large bacterium, was present in huge numbers (11.3 X 10(10) and 1.3 X 10(10) ml-1 after 73 days). Direct microscopic counts were also done on small bacteria, moderate-sized Selenomonas spp., and small Entodinium spp., which were the only protozoa seen. After the necessary dilution of rumen contents to make the microbial cells visible, Quin's ovals were seen to be much smaller in sheep 1 than in sheep 2. Most-probable-number estimates indicated that Methanobrevibacter spp. were present at 10(7) ml-1, Methanosarcina spp. were present at 10(3) ml-1, and Eubacterium limosum-like bacteria were present at 10(5) to 10(6) ml-1. In the adapted sheep, the dry portion of the diet was rapidly consumed, but the molasses mixture was consumed over a 9- to 10-h period. Volatile fatty acids in the rumen were present in very low amounts just prior to feeding and were much higher during the consumption of the diet, with about a 1:1 molar ratio of propionate to acetate between 1 and 9 h after feeding. Data were obtained on hourly feed consumption, levels of volatile fatty acids, and pH.(ABSTRACT TRUNCATED AT 250 WORDS)
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- Genthner B. R., Davis C. L., Bryant M. P. Features of rumen and sewage sludge strains of Eubacterium limosum, a methanol- and H2-CO2-utilizing species. Appl Environ Microbiol. 1981 Jul;42(1):12–19. doi: 10.1128/aem.42.1.12-19.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HUNGATE R. E. Mutualisms in protozoa. Annu Rev Microbiol. 1950;4:53–66. doi: 10.1146/annurev.mi.04.100150.000413. [DOI] [PubMed] [Google Scholar]
- MOIR R. J., MASSON M. J. An illustrated scheme for the microscopic identification of the rumen microorganisms of sheep. J Pathol Bacteriol. 1952 Apr;64(2):343–350. doi: 10.1002/path.1700640210. [DOI] [PubMed] [Google Scholar]
- Miller T. L., Wolin M. J., Zhao H. X., Bryant M. P. Characteristics of methanogens isolated from bovine rumen. Appl Environ Microbiol. 1986 Jan;51(1):201–202. doi: 10.1128/aem.51.1.201-202.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Orpin C. G. The culture in vitro of the rumen bacterium Quin's Oval. J Gen Microbiol. 1972 Dec;73(3):523–530. doi: 10.1099/00221287-73-3-523. [DOI] [PubMed] [Google Scholar]
- Paster B. J., Canale-Parola E. Treponema saccharophilum sp. nov., a large pectinolytic spirochete from the bovine rumen. Appl Environ Microbiol. 1985 Aug;50(2):212–219. doi: 10.1128/aem.50.2.212-219.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Prins R. A. Isolation, culture, and fermentation characteristics of Selenomonas ruminantium var. bryantivar. n. from the rumen of sheep. J Bacteriol. 1971 Mar;105(3):820–825. doi: 10.1128/jb.105.3.820-825.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rowe J. B., Loughnan M. L., Nolan J. V., Leng R. A. Secondary fermentation in the runen of a sheep given a diet based on molasses. Br J Nutr. 1979 Mar;41(2):393–397. doi: 10.1079/bjn19790048. [DOI] [PubMed] [Google Scholar]
- WARNER A. C. Some factors influencing the rumen microbial population. J Gen Microbiol. 1962 Apr;28:129–146. doi: 10.1099/00221287-28-1-129. [DOI] [PubMed] [Google Scholar]
- Wicken A. J., Howard B. H. On the taxonomic status of "Quin's oval" organisms. J Gen Microbiol. 1967 May;47(2):207–211. doi: 10.1099/00221287-47-2-207. [DOI] [PubMed] [Google Scholar]
- Ziołecki A. Isolation and characterization of large treponemes from the bovine rumen. Appl Environ Microbiol. 1979 Jan;37(1):131–135. doi: 10.1128/aem.37.1.131-135.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]