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Mammary-derived signals activate PCD during the first stage of involution. Histological analysis of mammary tissue from 3 days open (A and D) and closed glands (B and E) compared with normal 3 days involution (C and F). Hematoxylin and eosin staining (A, B, and C). In situ detection of PCD (D, E, and F). Arrows indicate cells undergoing PCD. Open, open teat; closed, Nexaband sealed teat; inv, normal involution after pup removal; 3d, 3 days; Lu, lumen.
