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. 1987 Jun;53(6):1316–1321. doi: 10.1128/aem.53.6.1316-1321.1987

Purification of the mosquitocidal and cytolytic proteins of Bacillus thuringiensis subsp. israelensis.

J M Hurley, L A Bulla Jr, R E Andrews Jr
PMCID: PMC203862  PMID: 3606108

Abstract

Two proteins from parasporal crystals of Bacillus thuringiensis subsp. israelensis were purified to electrophoretic homogeneity by gel filtration and anion-exchange chromatography. The larger of the two proteins (molecular weight, 68,000) was not cytolytic, whereas the smaller protein (molecular weight, 28,000) was highly cytolytic when assayed against rat erythrocytes. When these proteins were assayed against larvae of the yellow fever mosquito, Aedes aegypti, the larger protein was at least 100-fold more toxic than the smaller protein. Although proteolytic activity was not detected in solubilized crystals nor in purified protein preparations, the toxin (molecular weight, 68,000) was readily degraded to smaller, nontoxic molecules, even when maintained at 4 degrees C. Mixtures of the two purified proteins were significantly more toxic to mosquito larvae than was either protein alone. Thus, it is likely that both the mosquitocidal and the cytolytic protein play roles in the overall insecticidal action of the parasporal crystal produced by this bacterium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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