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B. nigra rRNA genes are dominant over B. rapa rRNA genes in both natural and synthetic lines of the tetraploid B. juncea. RNA purified from B. nigra (lane 5), B. rapa (lane 9), or B. juncea (lanes 6–8) was subjected to primer extension using the same primer. Only B. nigra transcripts were detected in the tetraploids. RNA from natural B. juncea was tested in lane 6; synthetic B. juncea lines derived from reciprocal crosses were tested in lanes 7 and 8.
