Skip to main content
. Author manuscript; available in PMC: 2008 Jun 20.
Published in final edited form as: Virology. 2007 Feb 21;363(1):91–103. doi: 10.1016/j.virol.2007.01.016

Figure 3. Flow cytometry analysis of uptake of anti-HIV-1 PNAPBS-MTD peptide conjugates.

Figure 3

CEM cells (0.5 × 106) were incubated with increasing concentrations of PNAPBS-Tat and PNAPBS-Penetratin for 1 min at 4°C and 37°C. After washing thoroughly with phosphate buffered saline solution, the cells were resuspended in RPMI media with 2% FCS. (A) Flow cytometry data at 37°C in the presence of propidium iodide (B) The percent of FITC-uptake per 104 cells as a function of the concentration of PNAPBS-MTD peptide conjugates; (C) The flow cytometry data after trypsin treatment for 10 min; (D) The percent uptake per 104 cells after trypsin treatment.