Figure 1.

Effects of C- and N-terminal deletions on functional expression of KAT1. Schematic representation of the KAT1 C (A) and N (B) termini (striped horizontal bars) and their putative regulatory sites [p, protein phosphorylation sites according to Pearson and Kemp (19); CAP, cyclic nucleotide binding site; MAP1b, microtubule-associated protein interaction site; CAP/MAP1b, open bars). The average charge of the C terminus calculated by summing the charges over a range of 18 residues at pH 7.2 is illustrated as shaded areas underlying the striped horizontal bar in A. Deleted regions are given by black and gray horizontal bars indicating nonfunctional or functional expression of the appropriate channel mutant, respectively.