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. 1997 Apr 1;94(7):3454–3458. doi: 10.1073/pnas.94.7.3454

Figure 5.

Figure 5

CMO expression in expanding and expanded spinach leaves. Plants had been irrigated with nutrient solution (lane 0) or for 10 days before the experiment with nutrient solution containing 200 mM NaCl (lane 200). (A) RNA blot analysis. Lanes contained 5 μg of total RNA. The probe was a 532-bp DNA fragment [positions 660–1,191 of pRS3 (Fig. 4)]. Ethidium bromide staining demonstrated that all lanes contained equivalent amounts of RNA. Densitometry of autoradiographs indicated that salinization increased CMO mRNA levels 2-fold in expanding leaves and 7-fold in expanded leaves. (B) Immunoblot analysis. Lanes contained 40 μg of total leaf protein. Rabbit antibodies against SDS-denatured CMO were used for immunodetection. (C) Enzyme activity. CMO was assayed following precipitation of leaf proteins with PEG 8000 (17). Bars are means of three determinations; SE values were ≤16% of the means.