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Nonheme iron and acid-labile sulfur content of CMO
| Analyte | nmol·ml−1 |
|---|---|
| CMO subunit | 57 (0.3) |
| FE | 105 (5.0) |
| S | 99 (6.0) |
Enzymatically active CMO was prepared as described (17). The reverse-phase HPLC elution profile (absorbance at 280 nm) of the preparation indicated that CMO was 51% of the total protein. This value, together with a Mr of 42,864 was used to calculate the molar concentration of CMO subunit. Values are means and SE (in parentheses) for three or four determinations.
