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. 1997 Apr 1;94(7):3459–3464. doi: 10.1073/pnas.94.7.3459

Figure 4.

Figure 4

Purification of P. syringae pv. tomato DC3000 extracellular appendages (flagella and Hrp pili). (A) Electron micrograph of the pellet fraction containing flagella and Hrp pili (indicated by an arrow). (B) Electron micrograph of a fraction from the middle of the gradient containing only flagella. (C) An SDS/15% PAGE gel of proteins from the pellet fraction (lane 1) or a fraction from the middle of the gradient containing only flagella (lane 2). The gel was stained with 0.025% Coomassie brilliant blue R-250. Lane M, molecular mass markers (Bio-Rad) in kDa.