Figure 1.

Restriction map of genomic clones of ACCase genes from B. napus. The organization of different ACCase gene classes is shown. Clones BnACCaseg3 and 8 are overlapping and represent the complete class I.1 gene (promoter and coding sequence). Restriction fragments hybridizing with the PCR probe used for gene isolation are indicated as gray bars. Regions analyzed by sequencing are shown as white bars interrupted by black boxes describing exons of the coding region or by a gray box pointing to an intron in the 5′ untranslated region (BnACCaseg1). Exons that are confirmed by sequencing of cDNA fragments generated by reverse-transcriptase–PCR are underlined. Positions of the identified start ATG and stop TGA codons as well as the MKM biotin binding motif are indicated. The “Y” at the ends of each clone symbolizes XbaI, SacI, NotI, and SalI restriction sites.