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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2007 Apr 14;1768(7):1703–1716. doi: 10.1016/j.bbamem.2007.04.007

Fig. 3.

Fig. 3

Determination of thermodynamic functions for unconjugated polyclonal rabbit anti-human fgn-fgn binding: ELISA Data. a. Semi-log dose-response curves at all three temperatures; circles, solid line: 4°C; circles, dotted line: 23°C; inverted triangles, dashed line: 37°C. b. Dose response at 4°C, hyperbolic fit [r = 0.990; y = 0.974x/(2.767 + x)]. c. Linear transform (reciprocal plot) of data from b, quadratic fit [r = 0.996; y = 0.860x2 + 0.045x + 1.518]. d. van't Hoff plot of corrected ELISA affinities.