Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2008 Jul 1.

Published in final edited form as: Biochim Biophys Acta. 2007 Apr 14;1768(7):1703–1716. doi: 10.1016/j.bbamem.2007.04.007

TABLE 1.

Unconjugated Anti-Fibrin(ogen)/Fibrinogen Binding Characteristics

RIA						ELISA
Temp. (°C)	K_assoc (M⁻¹)	No. Bind. Sites/Mol. Ab	ΔH° (kcal/mole)	ΔG° (kcal/mole)	ΔS° (cal/mole°)	Apparent K_assoc (M⁻¹)	“True” K_assoc (M⁻¹)	ΔH° (kcal/mole)	ΔG° (kcal/mole)	ΔS° (cal/mole°)
4	5.77×10⁸	0.77	−4.65	−11.10	+23.31	5.55 × 10⁷	8.59 × 10⁷	−1.05	−10.06	+32.50
4	6.06×10⁷	2.12	−11.93	−9.86	−7.46	5.55 × 10⁷	8.59 × 10⁷	−1.05	−10.06	+32.50
24/23	4.55×10⁸	0.44	−4.65	−11.77	+23.97	4.56 × 10⁷	7.80 × 10⁷	−1.05	−10.69	+32.55
24/23	2.02×10⁷	1.78	−11.93	−9.93	−6.73	4.56 × 10⁷	7.80 × 10⁷	−1.05	−10.69	+32.55
37	2.23×10⁸	0.49	−4.65	−11.84	+23.21	3.65 × 10⁷	7.00 × 10⁷	−1.05	−11.13	+32.50
37	5.80×10⁶	5.26	−11.93	−9.59	−7.53	3.65 × 10⁷	7.00 × 10⁷	−1.05	−11.13	+32.50