TABLE 2.
RIA | ELISA | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Temp.
(ºC) |
Kassoc (M−1) |
No.
Site/Lip. × 103 |
%
Tot. Ab |
ΔH°
(kcal/mole) |
ΔG°
(kcal/mole) |
ΔS°
(cal/mole°) |
Apparent
Kassoc (M−1) |
“True”
Kassoc (M−1) |
ΔH°
(kcal/mole) |
ΔG°
(kcal/mole) |
ΔS°
(cal/mole°) |
4 | 7.81 × 108 | 2.21 | 16.4 | −1.90 | −11.27 | +33.81 | 4.35±0.72
× 107 |
7.61±0.61
×107 |
−0.37±1.20 | −9.99
±0.04 |
+34.84
±4.42 |
4.23 × 106 | 103 | 766 | +5.99 | −8.40 | +51.92 | ||||||
26/20 | 5.13 × 108 | 2.65 | 19.6 | −1.90 | −11.92 | +33.48 | 6.60±3.20
× 107 |
9.15±2.16
×107 |
−0.37±1.20 | −10.66
±0.13 |
+35.24
±4.53 |
1.30 × 107 | 26.7 | 198 | +5.99 | −9.73 | +52.57 | ||||||
37 | 5.73 × 108 | 2.69 | 19.9 | −1.90 | −12.42 | +33.93 | 4.24±2.73
× 107 |
7.28±2.16
×107 |
−0.37±1.20 | −11.13
±0.17 |
+34.83
±4.41 |