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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2007 Apr 14;1768(7):1703–1716. doi: 10.1016/j.bbamem.2007.04.007

TABLE 3.

Unconjugated Monoclonal Anti-Fibrin(ogen)/Fibrinogen Binding Characteristics

RIA ELISA
Temp.
(ºC)		 Kassoc
(M−1)		 No. Bind.
Sites/Mol. Ab		 ΔH°
(kcal/mole)		 ΔG°
(kcal/mole)		 ΔS°
(cal/mole°)		 Apparent
Kassoc (M−1)		 “True”
Kassoc (M−1)		 ΔH°
(kcal/mole)		 ΔG°
(kcal/mole)		 ΔS°
(cal/mole°)
4 2.15×108 1.34 −7.53 −10.56 +10.95 8.42 × 108 3.23 × 108 −3.52 −10.78 +26.21
24/23 1.27×108 1.39 −7.53 −11.01 +11.74 4.91 × 108 2.48 × 108 −3.52 −11.37 +26.50
37 4.73×107 1.43 −7.53 −10.89 +10.84 2.02 × 108 1.61 × 108 −3.52 −11.64 +26.18