TABLE 4.
RIA | ELISA | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Temp.
(ºC) |
Kassoc (M−1) |
No.
Site/Lip. × 103 |
%
Tot. Ab |
ΔH°
(kcal/mole) |
ΔG°
(kcal/mole) |
ΔS°
(cal/mole°) |
Apparent
Kassoc (M−1) |
“True”
Kassoc (M−1) |
ΔH°
(kcal/mole) |
ΔG°
(kcal/mole) |
ΔS°
(cal/mole°) |
4 | 1.08 × 107 | 32.6 | 642 | +3.05 | −8.91 | +43.19 | 5.65 × 109 | 8.19 ×108 | +1.61 | −11.30 | +46.60 |
25/23 | 1.23 × 107 | 21.9 | 433 | +3.05 | −9.67 | +42.67 | 7.69 × 109 | 9.52 ×108 | +1.61 | −12.16 | +46.52 |
37 | 2.05 × 107 | 15.4 | 303 | +3.05 | −10.37 | +43.29 | 1.07×1010 | 1.12 ×109 | +1.61 | −12.84 | +46.60 |