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. Author manuscript; available in PMC: 2008 Jul 1.

Published in final edited form as: Biochim Biophys Acta. 2007 Apr 14;1768(7):1703–1716. doi: 10.1016/j.bbamem.2007.04.007

TABLE 5.

Monoclonal Anti-Fibrin(ogen) Fab/Fibrinogen Binding Characteristics – RIA Data

Unconjugated						Conjugated
Temp. (ºC)	K_assoc (M⁻¹)	No. Bind. Sites/Mol. Fab	ΔH° (kcal/mole)	ΔG° (kcal/mole)	ΔS° (cal/mole°)	K_assoc (M⁻¹)	No. Site/Lip. × 10³	% Tot. Ab	ΔH° (kcal/mole)	ΔG° (kcal/mole)	ΔS° (cal/mole°)
4	3.06×10⁸	0.23	−10.44	−10.75	+1.13	6.93 × 10⁷	46.2	89.8	−2.04	−9.94	+28.51
	3.06×10⁸	0.23	−10.44	−10.75	+1.13	1.35 × 10⁷	195	379	−2.22	−9.04	+24.61
	8.11×10⁷	0.38	−7.50	−10.02	+9.12	5.98 × 10⁶	377	733	−1.55	−8.59	+25.39
25/26	8.56×10⁷	0.21	−10.44	−10.82	+1.26	5.23 × 10⁷	62.0	121	−2.04	−10.56	+28.49
25/26	2.02×10⁷	0.43	−7.50	−10.12	+8.80	9.98 × 10⁶	233	454	−2.22	−9.60	+24.60
37	2.02×10⁷	0.35	−7.50	−10.36	+9.24	4.70 × 10⁷	59.0	115	−2.04	−10.88	+28.52
37	2.02×10⁷	0.35	−7.50	−10.36	+9.24	4.45 × 10⁶	425	826	−1.55	−9.43	+25.40