Table 1.
Summary of the main ultrastructural features of the subcellular and subsynaptic localization of NMDA and AMPA receptor subunits in the basal ganglia, as revealed by high resolution immunogold procedures.
| Structure | Technique | GluR1 | GluR2/3* | GluR4 | NR1 | NR2A/B* | Colocalization |
|---|---|---|---|---|---|---|---|
| Striatum (refs. 1, 2, 3) |
Pre- and postembedding (refs. 1,2 |
Synaptic Extrasynaptic (refs. 1,3) |
Synaptic Extrasynaptic (refs. 1,2,3) |
Restricted to interneurons Synaptic and extrasynaptic (refs. 1, 3) |
Synaptic Extrasynaptic (very few) (refs. 2,3) |
Synaptic (ref. 3) |
NR1 & GluR2/3 (ref. 2) |
| Postembedding (ref. 3) |
Presynaptic (ref. 3) |
Presynaptic (ref. 3) |
|||||
| Presynaptic (ref. 3) |
|||||||
| GP (ref. 2) |
Pre- and postembedding |
-- | Synaptic Extrasynaptic |
-- | Synaptic Extrasynaptic (very few) |
-- | NR1 & GluR2/3 |
| STN (ref. 4) |
Postembedding | Synaptic | Synaptic | Not detected | Synaptic | -- | GluR1 & GluR2/3, NR1 & GluR2/3 |
| Entopeduncular nucleus (EP, rodent analog of the GPi) (ref. 4) |
Postembedding | Synaptic | Synaptic | Synaptic | Synaptic | -- | GluR1 & GluR2/3 GluR4 & GluR2/3 NR1 & GluR2/3 |
| SNr (ref. 5) |
Pre- and postembedding |
-- | Synaptic Extrasynaptic |
-- | Synaptic Extrasynaptic |
Synaptic | NR1 & GluR2/3 NR1 & NR2 |
| SNc (ref. 5) |
Pre- and postembedding |
-- | Synaptic Extrasynaptic |
-- | Synaptic Extrasynaptic |
Synaptic | NR1 & GluR2/3 NR2 & GluR2/3 NR1 & NR2 |
--
Data not available
*
The immunolocalization of GluR2 and GluR3 subunits or NR2A/B was achieved using antibodies that recognize both subunits, providing no distinction in the localization of each respective subunits.
References: