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. Author manuscript; available in PMC: 2008 Jun 1.
Published in final edited form as: Cancer Cell. 2007 Jun;11(6):539–554. doi: 10.1016/j.ccr.2007.04.017

Figure 1. Purification of ECs from normal and malignant tissues.

Figure 1

A: Immunofluorescence staining of heart tissue demonstrated co-localization of CD105 (green) with VE-cadherin (red) in the vessels. Scale bar, 20 μm.

B: Immunofluorescence staining of liver tissue with CD105 (green). Scale bar, 20 μm.

C: A QPCR analysis was used to assess the purity of the EC preparations. QPCR analysis was performed on cDNA generated directly from unfractionated normal whole tissues (WT) or from purified ECs isolated from normal tissues (N-ECs) or the tumors (T-ECs) indicated. The endothelial-specific transcript VE-cadherin was enriched 110 to 530-fold in the endothelial fractions. The modest level of VE-cadherin found in the unfractionated heart and lung sample is presumably due to a higher proportion of ECs in these tissues. In this experiment, gene expression was normalized to that of the Eif4h, a gene found to be uniformly expressed in all cells as assessed by SAGE (Velculescu et al., 1999). Unfractionated brain was used to calibrate relative expression because this tissue had the lowest VE-cadherin expression levels.

D: Model used to identify genes expressed during pathological but not physiological angiogenesis. ECs were isolated from normal resting livers, regenerating livers, or tumor bearing livers.