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. Author manuscript; available in PMC: 2008 Jun 1.
Published in final edited form as: Cancer Cell. 2007 Jun;11(6):539–554. doi: 10.1016/j.ccr.2007.04.017

Figure 3. LEM and BEM genes identified by SAGE are expressed by ECs in vivo.

Figure 3

Localization of mRNA in ECs (red stain) is demonstrated for the brain endothelial markers GLUT-1 (BEM1) and organic anion transporter 2 (BEM2), and the liver endothelial markers deoxyribonuclease 1-like 3 (LEM1) and oncogene induced transcript 3 (LEM2). Note that the BEMs are selectively expressed in brain endothelium whereas the LEMs are selectively expressed in liver endothelium. The endothelial control probe, VEGFR2, stains both brain and liver endothelium. Staining of LEMs is most prominent in the sinusoidal endothelium, wherein the nuclear body appears to stain most intensely. A dilute counterstain was applied to the sections to highlight the lack of detectable expression in the non-ECs of the tumors. Scale bars, 50 μM