Table 1. SAGE tags elevated specifically in brain endothelium or liver endothelium.
The 27 genes which displayed more than 20-fold expression in brain endothelium compared to the 6 other normal endothelial libraries shown are listed in descending order. Likewise, 14 genes were found to be expressed at levels 20-fold higher in liver endothelium compared to the other normal endothelial libraries. To calculate tag ratios, we assigned a value of 0.5 in cases where zero tags were observed. Tag numbers for each group were normalized to 100,000 transcripts except for kidney which was normalized to 30,000 tags due to the small number of tags obtained for that particular tissue (Table S1). The tag sequences corresponding to the genes shown can be found in Tables S4 and S5. Genbank accession numbers and a description of the gene product corresponding to each tag are given.
| Brain | Heart | Kidney | Liver | Lung | Muscle | Spleen | Acc.# | Description* | |
|---|---|---|---|---|---|---|---|---|---|
| BEMs | |||||||||
| 1 | 754 | 8 | 1 | 2 | 1 | 12 | 4 | NM_011400 | GLUT-1† (Pardridge et al., 1990; Farrell et al., 1991) |
| 2 | 157 | 0 | 0 | 0 | 0 | 1 | 0 | NM_030687 | Organic anion transporter 2† (Gao et al., 2000) |
| 3 | 93 | 0 | 1 | 0 | 0 | 1 | 1 | NM_008973 | Pleiotrophin (Yeh et al., 1998) |
| 4 | 32 | 0 | 0 | 0 | 0 | 0 | 0 | NM_009728 | ATPase, class V, type 10A† |
| 5 | 40 | 0 | 0 | 0 | 1 | 0 | 0 | NM_009402 | Peptidoglycan recognition protein 1 |
| 6 | 26 | 0 | 0 | 0 | 0 | 0 | 0 | NM_021471 | Organic anion transp. 14† (Tohyama et al., 2004) |
| 7 | 29 | 0 | 0 | 0 | 0 | 0 | 0 | NM_008239 | Forkhead box Q1 |
| 8 | 19 | 0 | 0 | 0 | 0 | 0 | 0 | NM_031194 | Organic anion transporter 3† (Mori et al., 2003; Ohtsuki et al., 2004) |
| 9 | 73 | 0 | 0 | 0 | 3 | 0 | 0 | NM_172479 | SN2, Solute carrier family 38, member 5† |
| 10 | 40 | 0 | 0 | 0 | 1 | 2 | 0 | NM_172471 | Inter-alpha (globulin) inhibitor H5 |
| 11 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | NM_010703 | Lymphoid enhancer binding factor 1 |
| 12 | 23 | 0 | 0 | 0 | 0 | 0 | 1 | NM_011404 | Slc7a5 aa transporter† (Kageyama et al., 2000) |
| 13 | 20 | 1 | 0 | 0 | 0 | 0 | 0 | NM_023805 | Solute carrier family 38, member 3† |
| 14 | 17 | 0 | 0 | 0 | 0 | 0 | 0 | NM_009574 | Zinc finger protein of the cerebellum 2 |
| 15 | 81 | 6 | 0 | 0 | 1 | 3 | 0 | NM_052994 | Testican-2 (Schnepp et al., 2005) |
| 16 | 26 | 0 | 1 | 0 | 1 | 1 | 0 | NM_008256 | 3-HMG-CoA synthase 2 |
| 17 | 15 | 0 | 0 | 0 | 0 | 0 | 0 | NM_028748 | Progestin and adipoQ receptor family member V |
| 18 | 68 | 0 | 1 | 2 | 1 | 0 | 1 | AK172004 | APC down-regulated 1, Drapc1 |
| 19 | 13 | 0 | 0 | 1 | 0 | 0 | 0 | NM_027096 | Unknown‡, GDPD phosphodiesterase family |
| 20 | 26 | 0 | 0 | 3 | 1 | 0 | 0 | NM_029001 | Unknown‡, putative transmembrane protein |
| 21 | 19 | 1 | 0 | 0 | 0 | 1 | 0 | NM_027299 | DES2, lipid desaturase/ C4-hydroxylase |
| 22 | 39 | 0 | 1 | 0 | 2 | 0 | 1 | XM_486083 | Unknown‡, kelch repeat and BTB (POZ) domain |
| 23 | 46 | 2 | 1 | 0 | 1 | 1 | 0 | NM_017405 | Lipolysis stimulated receptor† |
| 24 | 36 | 2 | 0 | 0 | 1 | 1 | 0 | NM_010357 | Glutathione S-transferase, alpha 4† |
| 25 | 9 | 0 | 0 | 0 | 1 | 0 | 0 | NM_013869 | TNF receptor superfamily, member 19 |
| 26 | 17 | 1 | 0 | 0 | 0 | 1 | 0 | NM_011532 | T-box 1 |
| 27 | 6 | 0 | 0 | 0 | 1 | 0 | 0 | XM_620023 | Unknown‡, putative transmembrane protein |
| LEMs | |||||||||
| 1 | 0 | 0 | 0 | 196 | 0 | 0 | 0 | NM_007870 | Deoxyribonuclease 1-like 3 |
| 2 | 0 | 0 | 0 | 58 | 0 | 0 | 3 | NM_010959 | LZP, oncoprotein induced transcript 3 |
| 3 | 0 | 0 | 0 | 16 | 0 | 0 | 0 | NM_023438 | Unknown‡, putative transmembrane protein |
| 4 | 1 | 0 | 0 | 123 | 0 | 0 | 6 | AK150613 | CD32 (Muro et al., 1993) |
| 5 | 0 | 1 | 0 | 33 | 0 | 1 | 1 | NM_033616 | Unknown‡, putative G-protein coupled receptor |
| 6 | 0 | 1 | 0 | 14 | 0 | 0 | 0 | NM_019985 | C-type lectin-like receptor 2 |
| 7 | 0 | 0 | 0 | 216 | 0 | 0 | 24 | NM_029465 | Clec4g (LSECtin) (Liu et al., 2004) |
| 8 | 0 | 1 | 0 | 42 | 2 | 1 | 0 | NM_018797 | Plexin C1 |
| 9 | 0 | 1 | 0 | 9 | 0 | 0 | 0 | NM_011719 | Wnt9B |
| 10 | 1 | 0 | 0 | 16 | 1 | 0 | 0 | AK144596 | Unknown |
| 11 | 0 | 1 | 0 | 9 | 0 | 0 | 0 | NM_008092 | GATA-binding protein 4 (Dame et al., 2004) |
| 12 | 0 | 0 | 0 | 10 | 1 | 2 | 0 | AB049755 | MBL-associated serine protease-3 |
| 13 | 0 | 0 | 0 | 5 | 0 | 0 | 1 | NM_023132 | Renin binding protein |
| 14 | 0 | 0 | 0 | 16 | 1 | 2 | 1 | NM_144830 | Unknown‡, putative transmembrane protein |
| 15 | 1 | 0 | 1 | 11 | 0 | 0 | 0 | NM_011243 | Retinoic acid receptor, beta |
gene product has either a putative or established role in BBB function
Genes previously shown to be expressed in either brain or liver endothelium are followed by the associated references
uncharacterized gene with structural domains indicated