Table 1.

Primers for PCR and sequencing

Gateway primers	Sequence (5-3')	Use
Term attB2F	GGGGACAGCTTTCTTGTACAAAGTGGAAATCAGAACGCAGAAGCGGTC	Amplification of terminator
Term attB3R	GGGGACAACTTTGTATAATAAGTTGTTTAAACTGGCAGTTTATGGCGGGC	Amplification of terminator
Lux E attB2R	GGGGACCACTTTGTACAAGAAAGCTGGGTTCAACTATCAAACGCTTCGG	Amplification of lux reporter
GFP attB2R	GGGGACCACTTTGTACAAGAAAGCTGGGTGACACATTTATTTGTATAGTTC	Amplification of gfp reporter
GFPopt attB1F	GGGGACAAGTTTGTACAAAAAAGCAGGCTAGGAGGAATAAAAAATGAGTAAAGGCGAAGAAC	Amplification of gfp reporter
XylRA attB4F	GGGGACAACTTTGTATAGAAAAGTTGCGTTGACTTAACTAACTTATAG	Amplification of xylR PxylA
XylRA attB1R	GGGGACTGCTTTTTTGTACAAACTTGTTGA TTTAAGTGAACAAGTTTATC	Amplification of xylR PxylA
XylA attB4F	GGGGACAACTTTGTATAGAAAAGTTGAAACATTGAAATAAACATTTATTTTGTATATGATG	Amplification of PxylA
XylA attB1R	GGGGACTGCTTTTTTGTACAAACTTGTTGATTTAAGTGAACAAGTTTATC	Amplification of PxylA
LuxA attB1F	GGGGACAAGTTTGTACAAAAAAGCAGGCTAGGAGGACTCTCTATGAAATTTGGAAAC	Amplification of lux reporter
SASP attB4F	GGG GAC AAC TTT GTA TAG AAA AGT TGT TTA AAC GCT GGA ACC TTT TGT TCC CAA AAG	Amplification of PsspA
SASP attB1R	GGG GAC TGC TTT TTT GTA CAA ACT TGT TTT ATT TAG TAT GGT TGG GTT AAC TGG	Amplification of PsspA
attR3	GGGGCGTTTAAACAAGCTTGTAAAACGACGGCCAGTG	sequencing
PMEattR4	GGGTTTAAACAACTTTGTATAGAAAAGTTGAACGAG	sequencing
Ccdb Rev	AGGAAGGGATGGCTGAGGTC	sequencing
Cm R4-R3F	CAGGCGGGCAAGAATGTGA	sequencing