Figure 4.
Deletion of the UPF1 gene suppresses several sup45 phenotypes. The growth of the transformants of two isogenic strains, 1A-D1628 (sup45Δ) and 1-1A-D1628 (sup45Δ upf1Δ), selected on 5-FOA medium (Fig. 3A) was tested by plating 100, and 10-1 serial dilutions of overnight cultures (left to right) onto YPD plates at 37°C (A), onto YPD plates with 1 mg/ml paromomycin (B) and onto synthetic complete -adenine -leucine plates (C). The same serially diluted cultures were also spotted on YPD plates at 25°C (D) to estimate the total number of cells analyzed. Following sup45 mutations were tested: 102, 107 (nonsense) and 103 (missense).