Table 1.
Mutant allele | Nucleotide substitution | Putative destabilizing motifa | |
Position/ORF size | Codon changeb | ||
his7-1 | 229/1659 | AAA→TAA | 311caTTGATtTTaagttgtccaggtTCGATGaTTCa |
lys9-A21 | 605/1341 | TTA→TAA | ND |
trp1-289 | 403/678 | CAG→TAG | 436gtTTGATtCagaagcaggtgggacaggtgaactTtTgGATtggaacTCGATtTCTgactgggttggaagg |
a The downstream sequences were searched for the presence of DSE (TGYYGATGYYYYY) [15]. The numbers above the sequences indicate the 5' boundaries of putative DSE (underlined sequences) in the corresponding open reading frame (ORF); ND – not detected. b Codon changes in the case of each mutation are shown, nucleotide changes are underlined.