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. 2007 Sep 17;8:326. doi: 10.1186/1471-2164-8-326

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Copyright © 2007 Altincicek and Vilcinskas; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Quantitative real time RT-PCR analysis of transcriptional levels of selected genes in immune challenged and untreated larvae. The mRNA levels of selected genes in immune challenged animals (black bars) were determined and are shown relative to their expression levels in untreated animals (white bars). The transcriptional rates of eristalin and ET-AMP10 were found to be increased over 14 and 34 fold, respectively, in response to LPS injection. In contrast, the amount of actin and α-tubulin transcripts were not significantly influenced. Results represent mean values of three independent determinations ± S.D.