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Schematic of overall assay design. Briefly human cDNA is amplified in a non saturating PCR reaction possessing VB17 and Cy-5 labeled JB2.7-specific primers. Purified PCR products are combined with an equimolar mix of synthetic targets complementary to all 108 IRSS-specific CDR3β probes on the array and hybridized, washed and analyzed. Probe design is illustrated at the bottom of the figure.
