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. 2007 Sep 19;8:329. doi: 10.1186/1471-2164-8-329

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Copyright © 2007 Wang et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Hybridization of CDR3β-specific microarrays. Panel A. Cy3/FITC overlay image of Cy3-labeled A. thaliana LTP4 synthetic target (red) hybridized to 108 CDR3β-specific probes. Black arrows indicate negative controls, printing buffer with FITC-labeled tracking oligonucleotide (green) but no CDR3β-specific probe. Panel B. Cy3/FITC overlay image of Cy3-labeled IRSS clonotype 21 synthetic target (red, indicated by white arrow) hybridized to 108 CDR3β-specific probes. Outer columns of each subarray are negative controls. Note hybridization specificity.