Table 1.
Fatty acid composition in phospholipids of Caco-2 cells without (−) and with (+) cytokine stimulation (IL-1β and IFNγ) supplemented with different fatty acids (% of total fatty acids)
| Fatty acids | OA (160 μM) | ARA + OA (130 + 30 μM) | EPA + OA (6 + 154 μM) | ||||
|---|---|---|---|---|---|---|---|
| − | + | − | + | − | + | ||
| 16:0 (PA) | 16.0 | 16.1 | 18.7 | 19.5 | 16.3 | 15.8 | |
| 18:0 (SA) | 5.9 | 6.1 | 9.6 | 10.5 | 6.7 | 6.5 | |
| 18:1 trans | 3.0 | 3.1 | 6.1 | 5.7 | 2.7 | 2.7 | |
| 18:1 (n-7) | 3.4 | 3.4 | 2.6 | 2.5 | 3.3 | 3.3 | |
| 18:1 (n-9) (OA) | 47.1 | 46.3 | 14.4 | 13.8 | 45.2 | 45.0 | |
| 18:2 (n-6) (LA) | 1.7 | 1.7 | 1.1 | 1.1 | 1.7 | 1.6 | |
| 20:1 (n-9) | 2.1 | 2.1 | 0.3 | 0.3 | 1.7 | 1.7 | |
| 20:4 (n-6) (ARA) | 9.3 | 9.4 | 29.3 | 28.9 | 8.9 | 8.9 | |
| 20:5 (n-3) (EPA) | 0.4 | 0.4 | 0.0 | 0.0 | 2.1 | 2.2 | |
| 22:1 (n-9) | 0.8 | 0.8 | 1.5 | 1.3 | 0.8 | 0.8 | |
| 24:1 (n-9) | 2.0 | 2.1 | 0.9 | 1.0 | 2.0 | 2.1 | |
| 22:4 (n-6) | 0.4 | 0.4 | 7.7 | 7.8 | 0.3 | 0.3 | |
| 22:5 (n-3) | 0.5 | 0.5 | 0.4 | 0.4 | 1.3 | 1.3 | |
| 22:6 (n-3) (DHA) | 1.1 | 1.1 | 0.6 | 0.6 | 0.7 | 0.6 | |
| ∑ | 93.7 | 93.5 | 93.2 | 93.4 | 93.7 | 92.8 | |
| ∑ SAFA | 21.9 | 22.2 | 28.3 | 30.0 | 23.0 | 22.3 | |
| ∑ MUFA | 58.4 | 57.8 | 25.8 | 24.6 | 55.7 | 55.6 | |
| ∑ PUFA | 12.3 | 12.4 | 38.5 | 38.2 | 14.3 | 14.3 | |
| ∑ n-3 | 2.0 | 2.0 | 1.0 | 1.0 | 4.1 | 4.1 | |
| ∑ n-6 | 11.4 | 11.5 | 38.1 | 37.8 | 10.9 | 10.8 | |
| ∑ n-9 | 52.0 | 51.3 | 17.1 | 16.4 | 49.7 | 49.6 | |
Data are representative for two independent experiments and the data are derived from pooled samples
ARA arachidonic acid, EPA eicosapentaenoic acid, LA linoleic acid, MUFA monounsaturated fatty acids, OA oleic acid, PA palmitic acid, PUFA polyunsaturated fatty acids, SA stearic acid, SAFA saturated fatty acids, Σ sum, − non-stimulated, + cytokine stimulated