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. Author manuscript; available in PMC: 2007 Oct 22.
Published in final edited form as: Microb Pathog. 2006 Sep 6;41(6):207–217. doi: 10.1016/j.micpath.2006.07.006

Figure 3. Iha is expressed under iron-limiting conditions.

Figure 3

Iha expression was examined in outer membrane preparations of overnight cultures grown in iron-replete or iron-limiting conditions. 2 μg total outer membranes were separated by SDS-PAGE and transferred to nitrocellulose for immunoblot analysis (left) or stained with Coomassie blue (right).

(A): Addition of 10 μM FeSO4.7H2O to DMEM abolishes Iha expression in the outer membrane.

(B): Addition of the iron chelator 2,2′dipyridyl to LB results in expression of Iha in the outer membrane.