Table 1.
Strains and Plasmids
| E. coli strains | Description | Reference |
|---|---|---|
| 86-24 | EHEC O157:H7 | [18] |
| CFT073 | UPEC O6:K2:H1 | [19] |
| SM10λpir | λ/pir integrate with RP4 mob function | [50] |
| CC1005 | MC4100 pcnBTn10 DlacU169 araD139 rpsL thi | gift of Dr. Colin Manoil |
| BL21(DE3) | F-ompT hsdS gal dcm (DE3) | Novagen |
| 86-24nalR | [41] | |
| CFT073nalR | [15] | |
| ORN172 | [51] | |
| 86-24A3 | contains chromosomal iha-luc transcriptional fusion | this work |
| CFT073B5 | contains chromosomal iha-luc transcriptional fusion | this work |
|
| ||
| plasmids | Description | Reference |
|
| ||
| pIHA | iha in pBluescript | [8] |
| pUFRO47 | IncW replicon | [52] |
| pUC19Ω | omega fragment in pUC19 | [21] |
| pGPL01 | λ/pir-dependent origin of replication and promoterless luc | [40] |
| pRS415 | contains promoterless β-galactosidase and origin of replication from pBR322 | [43] |
| pEM42 | tet promoter drives expression of Renillaluciferase in pRL-Pnull | gift of Dr. Samuel Miller |
| pCRIICam | cat in AgeI site of pCRII | gift of Dr. Brad Cookson |
| pET21dFur | fur in pET21d | gift of Dr. T.V. O’Halloran |
| pGPL01R3 | iha sequence upstream of luc in pGPL01 | this work |
| pRL-PtetCm | cat from pCRIICam in Bam HI site of pEM42 | this work |
| pO157ihaP | 86-24 iha promoter driving β-galactosidase expression in pRS415 | this work |
| pCFTO73ihaP | CFTO73 iha promoter driving β-galactosidase expression in pRS415 | this work |
| pihaP-G/T | same as pO157ihaP but with "T" at −51 | this work |
| pihaP-A/C | same as pO157ihaP but with "A" at −85 | this work |