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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2007 Jan 31;1774(3):403–411. doi: 10.1016/j.bbapap.2007.01.002

Table 1.

Fluorescence parameter for ANS and 1NPN in buffer at pH 7.3

Fluorescence probe τ (ns) Q kr×10−7 (s−1) knr×10−8 (s−1) λmax(nm)
ANS 0.248 0.0032a 1.29 40.2b 540
1NPN 2.49 0.019 0.76 3.9b 475
a

Value is taken form references 10 and 15.

b

The intramolecular nonradiative decay rate is small in comparison to intermolecular electron-transfer rate in water. Therefore, this value is attributed fully to the intermolecular electron-transfer rate [10].