Figure 2.
(A) Coomassie-stained SDS-PAGE gel (B) Western Blot of wild type and mutant FMO3-expressing insect cell membranes. 10μg of insect cell membranes were loaded in each lane of a 9% acrylamide resolving gel. Lane 1, molecular weight markers in kDa; lanes 2 and 12, purified FMO3 standard; lane 3, uninfected insect cell membranes; lane 4, wild type FMO3; lane 5, N61S; lane 6, M66I; lane 7, P153L; lane 8, E158K; lane 9, E308G; lane 10, 158K/308G; lane 11, R492W.
