Table 1.
Oligonucleotide primers used in site directed mutagenesis of FMO3
| Mutation | Oligonucleotide primers |
|---|---|
| N61S |
5′-CAGTCTTTTCCAGCTCTTCC-3′
3′-GTCAGAAAAGGTCGAGAAGG-5′ |
| M66I |
5′-CCAAAGAGATTATGTGTTTCCC-3′
3′-GGTTTCTCTAATACACAAAGGG-5′ |
| P153L |
5′-GGACATCATGTGTATCTCAACCTACC-3′
3′-CCTGTAGTACACATAGAGTTGGATGG-5′ |
| E158K |
5′-CAACCTACCAAAAAAGTCCTTTCC-3′
3′-GTTGGATGGAAAATTCAGGAAAGG-5′ |
| E308G |
5′-CGTGAAGGAATTCACAGGGACCTCGGCC-3′
3′-GCACTTCCTTAAGTGTCCCTGGAGCCGG-5′ |
| 158K/308G |
5′-CAACCTACCAAAAAAGTCCTTTCC-3′
3′-GTTGGATGGAAAATTCAGGAAAGG-5′ 5′-CGTGAAGGAATTCACAGGGACCTCGGCC-3′ 3′-GCACTTCCTTAAGTGTCCCTGGAGCCGG-5′ |
| R492W |
5′-GGGACTGGTCGTTGAAAC-3′
3′-CCCTGACCAGCAACTTTG-5′ |