Table 2.
Characterization of FMO3 content in insect cell membrane preparations
| FMO3
Variant |
Immunoreactive
FMO3 content (nmol/mg) |
FAD content
(nmol/mg) |
Fraction
Holoprotein* |
|---|---|---|---|
| Wild Type | 1.36 ± 0.15 | 0.67 ± 0.01 | 0.49 |
| N61S | 1.52 ± 0.31 | 0.75 ± 0.01 | 0.49 |
| M66I | 2.30 ± 0.57 | 0.03 ± 0.01 | 0.01 |
| P153L | 0.74 ± 0.18 | 0.16 ± 0.01 | 0.21 |
| E158K | 2.19 ± 0.40 | 0.45 ± 0.01 | 0.21 |
| V257M | 1.72 ± 0.32 | 0.82 ± 0.04 | 0.48 |
| E308G | 2.53 ± 0.32 | 0.67 ± 0.02 | 0.27 |
| 158K/308G | 1.12 ± 0.05 | 0.65 ± 0.01 | 0.58 |
| R492W | 0.80 ± 0.15 | 0.06 ± 0.01 | 0.07 |
*
FAD content/immunoreactive FMO