Mobility-shift assays indicate that modified HMG-14 point mutants bind to nucleosome cores. Nucleosome cores containing either nonacetylated (n) or acetylated (ac) histones were incubated either without (lanes 1, 4, and 7) or with a 4-fold molar excess (lanes 2, 5, and 8) or an 8-fold molar excess (lanes 3, 6, and 9) of the modified HMG-14 point mutant indicated on the top of the lanes. C, position of free core particle; C+2, position of core containing two molecules of HMG-14 protein (see also Fig. 3).