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. Author manuscript; available in PMC: 2008 Aug 15.
Published in final edited form as: Exp Cell Res. 2007 Apr 27;313(14):3066–3075. doi: 10.1016/j.yexcr.2007.04.022

Figure 2.

Figure 2

Cell rounding on the nonadhesive substratum polyHEMA induces global histone deacetylation. (A) Phase contrast images of S1 cells cultured in 2D on tissue culture plastic or rounded on polyHEMA. Immunofluorescence images of AcH4 and DAPI staining in S1 cells cultured in 2D and on polyHEMA; (B) Western blot analysis of AcH3 and AcH4 in S1 and T4-2 cells cultured in 2D or on polyHEMA; (C) Bar graphs quantifying the relative AcH4 levels in S1 (n=4) and T4-2 (n=2) cells cultured in 2D and on polyHEMA. (**) p<0.01; (D) Quantification of nuclear DAPI staining in S1 cells cultured in 2D and on polyHEMA. (**) p<0.01; n=20; (E) Quantification of nuclear diameter for S1 cells cultured in 2D and on polyHEMA. (**) p<0.01; n=20.