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. 2007 Aug;91(4):335–342. doi: 10.1016/j.ymgme.2007.04.011

Fig. 3.

Fig. 3

Comparison of the effectiveness of different chaperones and combinations of I278T expressing yeast. Yeast (WY35pI278T) were diluted and growth was assessed identically to Fig. 2. Chaperone Set 1 contains 0.2 M sorbitol, 0.2 M TMAO, 0.44 M glycerol, 0.128 M DMSO. Chaperone Set 2 contains 0.4 M sorbitol, 0.4 M TMAO, and 0.2 M proline. Chaperone Set 3 contains 0.4 M sorbitol, 0.44 M glycerol, and 0.128 M DMSO. The lane labeled Control-WT shows growth of WY35phCBS in SC-cys media. The lane labeled Control on the right is WY35pI278T in SC-cys media.