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Degradation of polyadenylates by M. genitalium RNase R. RNase R activity on [H3]-poly(A) substrate was determined by acid soluble assay as described in Materials and Methods. Specific activities [in nanomoles of poly(A) per minute per milligram of enzyme] under various conditions are plotted as mean±standard error from three independent experiments. (A) pH range with 10 μM ZnCl2 and 100 mM KCl; (B) ZnCl2 or MgCl2, with 100 mM KCl at pH 8.5; (C) KCl, with 10 μM ZnCl2 at pH 8.5.
