FIGURE 3.

Degradation of oligoribonucleotides. Oligonucleotides were labeled at their 5′-ends with ³²P. Assays were carried out at 37°C in 40 μL reaction mixtures containing 25 μM of the indicated substrate; 1.0 μg of enzyme (EcR or MgR) was used in these analyses. Lanes labeled as “Buffer” indicate control reactions with no enzymes added. Five-microliter aliquots were taken at the times indicated, and the reactions were stopped with 2 volumes of RNA loading buffer. Products were resolved on 22.5% denaturing polyacrylamide gels. The sequences of the oligonucleotides used in this experiment are as follows: C4, CA14 (5′-CCCCACCACCAACA-3′), and C17.