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Rrp14p depletion causes a defect in assembly of 60S ribosomal subunits. (A) The polysome profiles from W303–1A transformed with pRS414 (WT) and KM1411 (GAL1–RRP14) strains cultured at 30°C in SCGal, shifted to SC, and cultured for the indicated time. (B) The polysome profiles from the KM1421 (rrp14Δ) strain cells cultured at 30°C in SC. The positions of 40S, 60S, and 80S ribosomal particles, and polysomes are indicated. Arrows indicate half-mer polysomes.
