FIGURE 7.

Rrp14p interacts with Utp11p, Faf1p, and Rps16p. Protein–protein interactions were analyzed by the yeast two-hybrid system. Fivefold serial dilutions of cultures of L40 strain cells transformed with lexA binding domain-fused (BD) and Gal4p activation domain-fused (AD) genes were plated on SC–Trp, Leu, and SC–Trp, Leu, His. As the empty vector containing lexA binding domain (pBTM116) exhibits a background level of expression, 1 mM 3-aminotriazole was added to a SC–Trp, Leu, His plate for the lower five combinations.