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Determination of k off rates of Phe- and Tyr-tRNAPhe from EF-Tu. The dissociation rates are (A) 0.21 ± 0.06 min−1 (Phe-tRNAPhe), 0.29 ± 0.19 min−1 (Tyr-tRNAPhe) at 2°C and (B) 7.7 ± 0.2 min−1 (Phe-tRNAPhe) and 6.8 ± 1.2 min−1 (Tyr-tRNAPhe) at 37°C. Data sets are the average of three independent experiments.
