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. 2007 Nov;13(11):1881–1886. doi: 10.1261/rna.684107

FIGURE 3.

FIGURE 3.

Decoding of Phe codons by Tyr-tRNAPhe and Phe-tRNAPhe. (A) fMet-Tyr and fMet-Phe dipeptide formation upon decoding of cognate (UUU and UUC) and near-cognate (CUU) Phe codons. Reactions contain 100 nM ribosomal initiation complex and 50 nM aa-tRNAPhe. The Y-axis shows the fraction of aa-tRNAPhe converted to dipeptide. Solid symbols, Tyr-tRNAPhe; open symbols, Phe-tRNAPhe. (B) Poly(U)-directed polyPhe and polyTyr synthesis. The reaction mixture contains 200 nM 70S ribosomes, 1 μM EF-G, 5 μM EF-Tu, 0.3 μg/μL poly(U) mRNA, and 1 μM [14C] Phe-tRNAPhe or [3H] Tyr-tRNAPhe synthesized with native E. coli tRNAPhe. As controls, 1 μM [14C] Phe or [3H] Tyr are added instead of [14C] Phe-tRNAPhe or [3H] Tyr-tRNAPhe. Data sets are the average of three independent experiments.