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Finding the “best” structural subgrouping. The clustering curve is plotted as total structure score (Sto) versus number of groups (G). The best subgrouping is found by finding the slope from the point at Sto(N) to the point that is halfway to the maximum Sto score, where N is the number of sequences in the alignment. The “best” subgroping is found by multiplying the slope by −1 and finding the point where it tangents the graph on the left side.
