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. Author manuscript; available in PMC: 2007 Dec 1.
Published in final edited form as: Glia. 2007 Dec;55(16):1668–1679. doi: 10.1002/glia.20574

Fig. 3.

Fig. 3

Confirming K+ conductance response in Kir4.1 expressing glioma cells and mature astrocytes. Representative ramp currents (−160 mV to +160 mV) are shown with an application of BaCl2 or 20 mM KCl solution. (A) D54-MG-Kir4.1AcGFP cells with BaCl2 blocked the Kir current similar to (B) DIV > 8 spinal cord astrocytes. Similar right shift of the reversal potential as well as an increased K+ conductance was observed after applying 20 mM KCl. The equivalent response was seen at DIV > 8 in vitro spinal cord astrocytes expressing Kir4.1. (C) Mock control D54-MG-AcGFP cells or (D) DIV < 5 immature astrocytes which do not express Kir4.1 had very small response to 100 μM BaCl2 and modest response to 20 mM KCl applications.