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. Author manuscript; available in PMC: 2007 Dec 1.
Published in final edited form as: Glia. 2007 Dec;55(16):1668–1679. doi: 10.1002/glia.20574

Fig. 7.

Fig. 7

Kir4.1 expression increases the percentage of cells in the G0/G1 phase of the cell cycle. FACS analysis was used to determine the relative percentage of cells found in each of the three discernable cell cycle stages. G0/G1 cells in (A) show an increased percentage of D54-MG-Kir4.1AcGFP cells in the G0/G1 phase. (B) Kir4.1 expressing cells showed a decrease in the G2/M phase without notable changes in S phase. (C) Application of 100 μM BA2+ or 20 mM high KCl solution revert this cell cycle change back to D54-MG-AcGFP control cells. (D) Significant reduction in % BrdU+ cell number is revealed in D54Kir4.1AcGFP cell line compared with D54-MG-AcGFP cells (P < 0.01). Treatment with 100 μM BA2+ or 20 mM KCl to D54-MG-Kir4.1-AcGFP cells restore % BrdU+ cell number similar to D54-MG-AcGFP mock transfected cells.