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. Author manuscript; available in PMC: 2007 Nov 1.
Published in final edited form as: Cell Biol Int. 2007 Jul 5;31(11):1301–1308. doi: 10.1016/j.cellbi.2007.06.011

Figure 4. Cholesterol exhibits no influence on the structure and assembly of the t-/v-SNARE complex.

Figure 4

(a) Full length recombinant v-SNARE in proteoliposomes, interact with full length recombinant t-SNARE-proteoliposomes in a circular array, to form t-/v-SNARE ring complexes. (b) Size of the t-/v-SNARE ring complex is directly proportional to the size of the proteoliposomes used. Note, that presence of cholesterol has no influence on the size, structure, and assembly of the t-/v-SNARE ring complex (a, b). (c) Resolution of Triton-Lubrol-solubilized proteoliposomes (PC:PS- or PC:PS:cholesterol-t-/v-SNARE ring complexes) on a sucrose gradient, demonstrates no difference in the presence or absence of cholesterol. (d) As observed for recombinant SNAREs (a-c), no difference in the native t-/v-SNARE ring complex is detected in presence of cholesterol.